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284 THE BLOOD.
nitrobenzene, pyrogallol, pyrocatechin, acetanilide, and certain other
bodies on the blood an abundant formation of methsemoglobin takes place.
By the action of light, Hasselbach,1
especially by the use of rays hav-
ing a wave-light below 310 n n, obtained methsemoglobin from oxyhemo-
globin, but not from haemoglobin in the absence of oxygen, and by this
behavior pure methsemoglobin can be prepared.
According to the investigations of Hufner, Kulz, and Otto 2
methsemoglobin contains just as much oxygen as oxyhsemoglobin, but
it is more strongly combined, a view which is accepted by most investiga-
tors. According to Hufner and v. Zeynek we can admit in the methsemo-
globin formation of an expulsion of oxygen and a combination of two
,OH
hydroxyl groups; methsemoglobin would then be Hb<^ . Accord-
NOH
ing to others, Hoppe-Seyler, Kuster, Letsch the methsemoglobin
contains less oxygen than the oxyhsemoglobin and is HbO or HbOH.
A methsemoglobin solution is converted into a haemoglobin solution
by reducing substances. The reaction taking place in the forma-
tion of methsemoglobin from oxyhsemoglobin by the action of potassium
ferricyanide has been quantitatively followed by v. Reinbold.3 He
found that one molecule of K3Fe(CN)6 was necessary to transform 1
molecule oxyhsemoglobin or to drive off 1 molecule of oxygen from the
oxyhsemoglobin. The reaction takes place according to the equation:
/°
Hb< |
+K3Fe(CN)6 -|-H20 = Hb.OH+K3HFe(CN)6+02
xO
and from his investigations he gives the formula Hb.OH to methsemo-
globin, in correspondence to the views of Kuster.
According to Hufner and Reinbold 4
1 gram methsemoglobin can
take up 2.685 cc. nitric oxide.
Methsemoglobin crystallizes, as first shown by Hufner and Otto,
in brownish-red needles, prisms, or six-sided plates. It dissolves easily
in water; the solution has a brown color and becomes a beautiful red
on the addition of alkali. The solution of the pure substance is not
precipitated by basic lead acetate alone, but by basic lead acetate and
1
Bioch. Zeitschr., 19.
*See Otto, Zeitschr. f. physiol. Chem., 7; v. Zeynek, Arch. f. (Anat. u.) Physiol.,
1899; Hufner, ibid.
3
Kuster, Zeitschr. f. physiol. Chem., 66; Letsche, ihid., 80; v. Reinbold, Zeitschr.
f. physiol. Chem., 85.
4
Arch. f. (Anat. u.) Physiol., 1904, Suppl.
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