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290 THE BLOOD.
and v. Zeynek 1
from haemoglobin and pyridine by boiling, or from
haematin and haemin and pyridine after the addition of hydrazin-hydrate.
Haemochromogen also combines, as Hoppe-Seyler first showed, with
carbon monoxide. This compound, which in aqueous solution gives
a spectrum similar to oxyhemoglobin, has been obtained by Pregl 2
in the solid condition as a deep-violet powder which is insoluble in
absolute alcohol. In opposition to haemoglobin the haemochromogen
combines with oxygen more firmly than with carbon monoxide. The
assumption of Hoppe-Seyler, that this compound is a combination of 1
molecule haemochromogen and therefore contains 1 molecule carbon
monoxide for 1 molecule of iron has been experimentally substantiated
by Hufner and Ruster and by Pregl.3
An alkaline haemochromogen solution has a beautiful cherry-red
color. It shows two absorption-bands, first described by Stokes (spec-
trum Plate, 6), one of which is dark and whose center corresponds to
X = 556.4 between D and E, and a second broader band, less dark, which
covers the Fraunhofer lines E and b. The middle of this band cor-
responds to X = 526 to 530 according to Lewin, Miethe and Stenger.
In acid solution haemochromogen shows four bands, which, according
to Jaderholm,4
depend on a mixture of haemochromogen and haemato-
porphyrin (see below), this last formed by a partial decomposition
resulting from the action of the acid.
Milroy,5
from an alcoholic solution of haematin containing oxalic
acid, after driving out the air by means of hydrogen gas, gradually obtained
an acid solution of reduced haematin (haemochromogen) by means of
zinc dust. This solution showed one absorption-band between D and E.
Haemochromogen may be obtained as crystals by the action of caustic
soda on haemoglobin at 100° C. in the absence of oxygen (Hoppe-Seyler).
By the decomposition of haemoglobin by acids (of course in the absence
of air) we obtain haemochromogen contaminated with a little haemato-
porphyrin. An alkaline haemochromogen solution is easily obtained by
the action of a reducing substance (Stokes’ reduction liquid) on an
alkaline haematin solution. An ammoniacal solution of haematin on
reduction with hydrazine yields haemochromogen very easily. An alco-
holic, alkaline hydrazine solution is also recommended by Riegler 6
as a reagent for blood-pigments, converting them into haemochromogen.
Haematin, also called Oxyh^ematin, is sometimes found in old transu-
dates. It is formed by the action of the gastric or pancreatic juices on
1
E. Kalmue, Zeitschr. f. Chem., 70; v. Zeynek, ibid., 70.
2
Ibid., 44.
• Hufner and Kiister, Arch. f. (Anat. u.) Physiol., 1904, Suppl. Pregl, 1. c.
* Nord. Med. Arkiv., 16.
5
Journ. of Physiol., 32.
8
Zeitschr. f. anal. Chem., 43.
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