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300 THE BLOOD.
An alcoholic solution of heematoporphyrin, acidulated with hydro-
chloric or sulphuric acid, shows two absorption-bands (spectrum Plate,
7), one of which is fainter and narrower and lies between C and D, near
D. The other is much darker, sharper, and broader, and lies midway
between D and E. An absorption extends from these bands toward
the red, terminating with a dark edge, which may be considered as a
third band between the other two.
A dilute alkaline solution shows four bands, namely, a band between
C and D; a second, broader band surrounding D and with the greater
part between D and E; a third, between D and E, nearly at E; and
lastly, a fourth, broad and dark band between b and F. On the addi-
tion of an alkaline zinc-chloride solution the spectrum changes more
or less rapidly,1
and finally a spectrum is obtained with only two bands,
one of which surrounds D and the other lies between D and E. If an
acid haematoporphyrin solution is shaken with chloroform, a part of the
pigment is taken up by the chloroform, and this solution often shows a
five-banded spectrum with two bands between C and D. The position
of the hsematoporphyrin bands in the spectrum differ with the various
methods of preparation and other conditions, so that they do not cor-
respond to the same wave length. These facts coincide well with the
recent investigations of A. Schulz;2
according to which the appearance
of the spectrum is not only dependent upon the reaction but also upon
the character of the solvent and the method of preparation.
In regard to the preparation of hsematoporphyrin, see Hoppe-Seyler-
Thierfelder’s Handbuch, 8. AufL, and the works cited on page 294.
Mesoporphyrin, C34H38N4O4, has the same spectrum as haematoporphyrin.
It has two oxygen atoms less, and further differs from it in that on oxidation it yields
hsematinic acid as well as methylethylmaleic imide, and does not show the
above-mentioned biological action of haematoporphyrin.
Haematinogen is a ferruginous pigment so named by Freund,3
which he
obtained by carefully extracting blood with alcohol containing hydrochloric
acid. It is closely related to haematin, but is not sufficiently characteristic and is
not considered as a cleavage product.
A question of great interest is whether it is possible to produce the
blood-pigment from its cleavage products. In this respect certain recent
investigations are interesting. Zaleski obtained from mesoporphyrin
hydrocloride dissolved in 80 per cent acetic acid saturated with NaCl
and heated to 50°-70°, a hsemin-like pigment by the addition of a solu-
tion of iron in acetic acid, and this pigment had a spectrum in acid
solution very similar to that of haematin, although not identical with it.
1
See Hammarsten, Skand. Arch. f. Physiol., 3, and Garrod, Journ. of Physiol., 13.
2
Arch. f. (Anat. u.) Physiol., 1904, Suppl.
3 Wien. klin. Wochenschx., 1903.
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