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364 CHYLE, LYMPH, TRANSUDATES AND EXUDATES.
The ash of pus-serum has the following i
composition, calculated to
1000 parts of the serum:
i. ii.
NaCl 5.22 5.39
Na2SO< 0.40 0.31
Na2 HP04 0.98 0.46
Na2C03
0.49 1.13
Ca3 (P04 ) 2 0.49 0.31
Mg3 (P04 ) 2 0.19 0.12
P04 (in excess) 0.05
The pus-corpuscles are generally thought to consist chiefly of emi-
grated white blood-corpuscles, and their chemical properties have there-
fore been given in discussing these. The molecular granules, fat-
globules, and red blood-corpuscles are considered rather as casual form-
elements.
The pus-cells may be separated from the serum by centrifugal force,
or by decantation directly or after dilution with a solution of sodium
sulphate in water (1 vol. saturated sodium-sulphate solution and 9 vols,
water) and then washed by this same solution in the same manner as
the blood-corpuscles.
The chief constituents of the pus-corpuscles are proteins, of which
the largest portion seems to be a nucleoprotein which is insoluble in
water and which expands into a tough, slimy mass when treated with a
10-per cent common-salt solution. This protein substance, which is
soluble in alkali but is quickly changed thereby, is called Rovida’s hyaline
substance, and the property of the pus of being converted into a slime-
like mass by a solution of common salt depends on this substance. Besides
this substance, to which the nucleoprotein of the pus-cells investigated
by Strada 1
seems to stand in close relation, we also have a globulin
which coagulates at 48-49° C, as well as serglobulin (?), seralbumint
a substance similar to coagulated protein (Miescher), and lastly peptone
or proteose (Hofmeister 2
). It is very remarkable that no nucleo-
histone or histone has been detected in the pus-cells, although histone
occurs in the cells of the lymph glands.
There are also found in the protoplasm of the pus-cells, besides the
proteins, lecithin, cholesterin, glucolhionic acid,3 purine bodies, fat, and soaps.
Hoppe-Seyler has found cerebrin, a decomposition product of a pro-
tagon-like substance, in pus (see Chapter XI). Kossel and Freytag 4
have isolated from pus two substances, pyosin and pyogenin, which
1
Bioch. Zeitschr., 16.
2
Miescher in Hoppe-Seyler’s Med.-Chem. Untersuch., 441; Ch. Pone. Maly’s Jahresb.,
39; Hofmeister, Zeitschr. f. physiol. Chem., 4.
3
Mandel and Levene, Bioch. Zeitschr., 4.
Zeitschr. f. physiol. Chem., 17, 452.
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