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810 URINE.
ylamine solution allowed to flow in from a burette until the blue color |
disappears and the solution is colorless, or, in urine poor in sugar, is yellow.
The sugar in milligrams is directly obtained from the amount of hydroxyl-
amine solution used by referring to the following reduction table: 4
Hydroxyl- Hydroxyl- Hydroxyl- Hydroxyl-
amine Milligrams amine Milligrams amine Milligrams amine. Milligrams
solution sugar. solution sugar. solution sugar. solution sugar.
used. used. used. used.
0.75 60.0 13.00 39.0 25.50 2300 | 38.00 10.4
1.00 59.4 13.50 38.3 26.00 22.9 38.50 9.9
1.50 58.4 14.00 30.7 26.50 BPA? 39.00 9.4
2.00 oto 14.50 BY 5 il 27.00 21.8 39.50 9.0
2.50 56.2 15.00 36.4 27.50 212 40.00 8.5
3.00 55.0 15.50 35.8 28.00 2007 40.50 8.1
3.50 54.3 16.00 Son Li 28.50 20.1 41.00 7.6
4.00 53.4 16.50 34.5 29.00 19.6 41.50 ta2
4.50 52.6 17.00 33.9 29.50 19.1 42.00 Gad
5.00 D6 17.50 33.3 30.00 18.6 42.50 6.3
san) 50.7 18.00 32.6 30.50 18.0 43.00 5.8
6.00 49.8 18.50 32.0 31.00 Ug G5) 43.50 5.4
6.50 48.9 19.00 31.4 31.50 17.0 44.00 4.9
7.00 48.0 19.50 30.8 32.00 16.5 44.50 4.5
7.50 47.2 20.00 30.2 32.50 15.9 45.00 4.1
8.00 46.3 20.50 29.6 33.00 15.4 45.50 Bf
8.50 45.5 21.00 29.0 33.50 14.9 46.00 3.0
9.00 44.7 21.50 28.3 34.00 14.4 46.50 2.9
9.50 44.0 22.00 Puff st 34.50 13.9 47.00 P48)
10.00 43.3 22.50 2M 35.00 13.4 47.50 Papal |
10.50 42.5 23.00 26.5 35.50 12.9 48 .00 Mei
11.00 41.8 2) 50) 25.8 36.00 12.4 48 .50 153)
11.50 41.1 24.00 DED 36.50 11.9 49.00 0.9
12.00 40.4 24.50 24.6 37.00 11.4
12.50 39.7 25.00 24.1 37.50 10.9
For every yo cc. hydroxylamine solution used more than given in the table
between 49.00-15.00, subtract 0.1 milligram from the corresponding sugar value
and 0.2 milligram between 15.00-1.0.
The yellow color of the urine may be somewhat disturbing for the
end reaction so that with little experience an error of 0.5 cc. hydroxylamine
solution (=about 0.5 milligram sugar) may occur. In order to decolorize
the urine we can precipitate, according to ANDERSEN, with mercuric
nitrate, when the greatest part of the disturbing reducing substances are
removed, and then the excess of mercury removed by caustic soda and
shaking with zinc. Still simpler is the suggestion mentioned on page
805 with blood-charcoal after acidification with acetic acid.
Bane” decolorizes by the addition of 2 cc. alcohol of 95-97 per cent
and a teaspoonful of blood-charcoal to 18 cc. urine, shaking and filtering
immediately. By this means 50 per cent of the other reducing substances
1 This table is given with the permission of the publisher, Julius Springer, Berlin,
where it can be obtained at a low cost.
2 Andersen, Bioch. Zeitschr., 15 and 37; Bang, ibid., 38.
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