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5
soil and air. Human faeces were collected ex ano in sterilized
widemouth 150 c. c. bottles. The samples af animal faeces tested
had in most cases to be collected on the floor of the cage, but
precautions were taken to avoid contamination as much as possible.
Only fresh dung, a few minutes old at the most, was used. The
outer particles were removed and with a scalpel repeatedly flamed
over an alcohol lamp the central parts of the excrements were dug
out and put in sterilized bottles. In order to obtain satisfactory
emulsions approximately 5 grams of the faeces were shaken for
10 minutes in 50 c. c. of distilled water. A few cubic centimetres
of this emulsion were poured into test tubes containing the media,
shaken, and then plated. If the samples were known to contain
an abundance of spores the procedure was somewhat different.
A few cubic centimetres of the spore-containing emulsion were trans-
ferred from the first test tubes into a second containing the media,
and again after vigorous shaking a few drops were transferred from
the second to a third. The contents of these tubes were then
transferred to Petri dishes. By this method the spores were
distributed singly, and a pure culture from a single spore was
readily obtained. The Petri dishes obtained from the same sample
of faeces were divided into two lots; one was incubated at 37? C.
for 12—24 hours, the other was kept at room temperature for
24—48 hours. The plates were then examined under a dissecting
microscope, and transfers were made to test tubes containing the
ordinary media.
3. Results of the Isolations.
Samples of faeces were obtained from 13 different persons, two
of them being vegetarians. Whenever possible, several samples
from the same person were examined. The date of examination
is given in every case.
Mixed diet A.
March 2, 1914: Aspergillus fumigatus, A. nidulans, Mucor.’
March 6: Aspergillus fumigatus, A. niger.
Mixed diet C.
Jan. 6, 1915: None; Jan. 13: Yeasts; Feb. 15: None; March 3:
None.
Vegetarian E.
March 2, 1914: Aspergillus terreus, A. niger, A. umbrinus.
* The culture was lost by an accident before any further determination had
been made.
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