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274 THE BLOOD.
When the haemoglobin is separated from the so-called stroma by a
sufficiently strong dilution with water the stroma is found in the solution
in a swollen condition. By the action of carbon dioxide, by the careful
addition of acids, acid salts, tincture of iodine, or certain other bodies,
this residue, rich in proteins, condenses, and in many cases the form of
the blood-corpuscles may be again obtained. This residue, the so-
called ghosts or stromata of the blood-corpuscles, can also be directly
colored in dilute blood by methyl violet and in this way detected, and
attempts have been made to isolate it for chemical investigation. In
the following pages we mean by the name stroma only that residue which
remains after the removal of haemoglobin and other bodies soluble in
water.
To isolate the stromata from the blood-corpuscles, they are washed
first by diluting the blood with 10-20 vols, of a 1-2 per cent common
salt solution and then separating the mixture by centrifugal force or
by allowing it to stand at a low temperature. This is repeated a few
times until the blood-corpuscles are freed from serum. These purified
blood-corpuscles are, according to Wooldridge, mixed with 5-6 vols,
of water and then a little ether is added until complete solution is obtained.
The leucocytes gradually settle to the bottom, a movement which may
be accelerated by centrifugal force, and the liquid which separates there-
from is very carefully treated with a 1-per cent solution of KHSO4 until
it is about as dense as the original blood. The separated stromata are
collected on a filter and quickly washed. Pasctjcci, 1
on the contrary,
treats the mass of corpuscles with 15-20 vols, of a one-fifth saturated
ammonium-sulphate solution, allows the corpuscles to settle, siphons
off the fluid, repeatedly centrifuges, allows the residue to dry quickly
(on porcelain plates) at the ordinary temperature, and then washes
with water until the blood-pigments and the other soluble bodies are
dissolved out.
Wooldridge found as constituents of the stromata lecithin, choles-
terin, nucleoalbumin, and a globulin which, according to Halliburton,
is probably a nucleoproteid which he calls cell-globulin. No nuclein
substances or seralbumin or proteoses could be detected by Hallibur-
ton and Friend. According to Pascucci, the stromata (from horse-
blood) consists of one-third cholesterin and lecithin (besides a little
cerebroside), and two-thirds protein substances and mineral bodies.
The nucleated red blood-corpuscles of the bird contain, according to
Plosz and Hoppe-Seyler,2
a protein (nucleoprotein) which swells to a
slimy mass in a 10-per cent common salt solution, and which seems to
1
Hofmeister’fi Beitrage, 0.
* Wooldridge, Arch. f. (Anat. u.) Physiol., 1881,387; Halliburton and Friend,
Journal of Physiol., 10; Halliburton, ibid., 18; P16sz, Hoppe-Seyler’s Med. chem.
Untersuch., 510.
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