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19
veterinarians under the name of epizootic cerebro-spinal meningitis,
are supposed to be caused by moulds in the fodder (PAMMEL 47).
In the following an account is given of the feeding experiments
which were performed in order to reach a definite conclusion as to
the toxic properties of moulds. The results of the isolations set
forth in an earlier part of this paper, show that the presence of
fungous spores in the digestive apparatus was of no rare occurrence
and, furthermore, that an accumulation in the alimentary canal of
fungous spores in the intestine likely took place in some instances,
judging by the quantity of fungi isolated. The serious consequences
which may result from an undue accumulation in the alimentary
canal of fungous spores with toxic properties are apparent and it
became the object of this experimental study to prove or disprove
the toxicity, in particular of those moulds which had been isolated
from the digestive tract of man.
The moulds to be tested were grown in slanted 100 x20 mm.
agar-agar tubes. They were kept in incubator at 37° C. for two
weeks, at the end of which time the tubes were partly filled with
mycelium and spore masses. This material was then smeared on
fresh spinach leaves and given the rabbits to eat.
RABBIT v, weight, 400 grams, fed on Penicillium divaricatum.
April 9, two full tubes given. April 12, four additional. April 14,
Exitus.
RABBIT VI, weight, 372 grams, fed on Aspergillus umbrinus. April
9, two full tubes given. April 12, four additional. April 14,
Exitus.
Postmortem examination of RaBBiTS v and vi showed in both
cases the stomach and caecum filled with partly digested food,
while the small intestine was practically empty. Lungs and kidneys
were of normal appearance and no fungous growth was to be
discovered in these organs or on the mucous membrane of the
stomach and intestine. Platings from the stomach and from the
large and small intestine demonstrated the abundance of spores all
along the alimentary canal.
RABBIT vi, weight, 423 grams, fed on Penicillium avellaneum.
April 12, four tubes. April 20, five tubes. April 21, Exitus.
Postmortem examination revealed the same characteristics of the
digestive apparatus as in RABBITS v and vi, and the fungus was
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