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22
and scratched the longue and the abdomen. June 28. The symp-
toms of disease were more striking. They were uneasy and rest-
less; pushed the abdomen against the bottom of the cage, raised
themselves on their hind legs and tried in vain to fly. The ab-
domen had taken on a darker color”.
June 29. Great drowsiness. No sound when the cage was given
a knock. They did not pay any attention to pure honey that was
given them. June 31. New honey and fungous material was given
in the same quantities as before. They ate with good appetite,
became wild and ran around as intoxicated. Some had in the
evening distinctly swollen abdomens. July 1. Two dead, with
swollen, black and glossy abdomens. July 3. Eleven bees dead;
the rest very drowsy and hardly able to move. July 4. All dead.
When dissected ventriculus and particularly rectum were found
to be dilated and swollen. The most swollen could hardly be touch-
ed without spurting out a green yellowish liquid, which received
its color mainly from the masses of spores of Penicillium sp. pre-
sent in the fluid. The contents of ventriculus and rectum were
plated and the fungus was recovered from both. i
Series Il.
>
July 25. 12 bees were caged. 0.1 gram fungous material was
mixed in 10 e. c. honey. July 24. One bee sick having swollen
abdomen. July 25. None of the bees dead, but one showed the
symptoms of the disease very plainly. It hopped along trying to
fly but the abdomen was too heavy and swollen. It sometimes
flapped the wings vigorously. The rest were rather lively. July 26.
One dead: several had swollen abdomens and staggering walk.
July 27. Seven of the bees dead, all of them swollen. The rest
apparently sick. July 28. One more dead; the rest hopping
around with great difficulty. July 30. All dead.
Penicillium stoloniferum Thom.
Series I.
June 27. 18 bees were caged. 0.5 grams of spores and myce-
lium were mixed in 10 c. c. honey. June 28. The same symptoms
?) The abdomen becomes in many instances entirely shiny and glossy when the
disease has advanced for some time. This is surely due to the fact that the bands
of down have been worn off by the incessant scratching of the abdomen with the
hind legs.
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