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24
ing to climb the walls of the box they fell down unfailingly. When
falling on the back great exertions had to be made to get on the
feet. They often raised themselves on the hind legs trying to fly,
but the weight of the swollen abdomen made flight impossible and
they usually fell on the back. July 15. Nine ofthe bees dead; the
rest apparently much affected. July 16. The rest dead.
Series. II,
July 17. 9 bees were caged. 0.4 grams of mycelium and spores
were mixed in 5 c. c. honey. July 18. A few were affected and
had black and swollen abdomens. July 19. Four dead, the ab-
domens swollen and glossy black. Three more dying. July 21. All
of them dead.
Series III.
July 25. 12 bees were caged. 0.1 gram fungous material was
mixed in 10 c. c. honey. July 24. No sure signs of disease could be
detected. July 25. One dead; several drowsy and swollen. July
26. Nine dead, greatly swollen. The remaining swollen and
drowsy, falling on the back when trying to climb the walls of the
box. July 27. All dead.
The bees poisoned with Mucor Mucedo were usually very much
swollen. Dissection showed both ventriculus and rectum dilated
and filled with spores of the fungus. In many cases germination
had taken place, the spores having put forth germtubes of consi-
derable length.
Cladosporium herbarum (Pers) Link.
July 23. 12 bees were caged. 0, gram fungous material was
mixed in 10 c. c. honey. July 24. No signs of disease could be
detected. July 25. One be dying. July 26. Four dead, rather
swollen. (Two were found drowned in the honey). July 27. One
more dead; the remaining very drowsy. July 28. One more dead;
three dying showing very characteristic signs of paralysis. They
crawl along with great difficulty on account of the swollen abdo-
men, fall on the side stretching the free hind leg fumbling in the
air and scratching the abdomen from time to time. One of them
shows powerful trembling of wings and body. July 29. All dead.
No germination of the spores had taken place in the body of the
bees.
nmm
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